Figure 4
Comparison of NiV M release with SV5 M and kinetics of NiV M release. (A) Culture supernatants were analyzed for release of either NiV or SV5 M. Released protein was treated as described in Methods and proteins were immunoprecipitated using either rabbit anti-NiV polyclonal serum or MAb M-h (anti-SV5 M). Image shown is over-exposed to show contrast. (B) Cells expressing NiV M were starved for 45 min, 35S-pulsed for 15 min, then chased with DMEM-10 for times indicated. Released protein was purified and protein derived from lysate or supernatant was immunoprecipitated with MAb F45G5. Percent protein release was quantitated by densitometry. Proteins derived from cell lysates (L) or culture supernatants (S) are indicated. Lysate bands represent 1/6 of total lysate.