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Figure 4 | Virology Journal

Figure 4

From: The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay

Figure 4

Caesium chloride isopycnic density-gradient ultra-centrifugation of the material eluted in the Vi of the gel filtration column. Freeze-dried material eluted in the Vi was dissolved in 4 M GuHCl containing 10 mM EDTA, 5 mM NEM and 0.05% CHAPS pH 6.5 and adjusted to a density of 1.39 to 1.40 g/ml with caesium chloride. Density gradient centrifugation was performed in a Beckman L45 ultra-centrifuge for 48 h at 105 000 g at 4Ā°C. Fractions with high PAS (ā—†) and low protein (Lowry) (ā– ) (a) were pooled and adjust to a density of 1.39 to 1.40 g/ml with caesium chloride for a second step centrifugation (b). Mucin rich fractions with a density (ā–²) between 1.37ā€“1.42 were pooled, dialysed against three changes of distilled water for overnight at 4Ā°C and freeze-dried.

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Virology Journal

ISSN: 1743-422X