Figure 4

Activation-induced EGFP expression in Jurkat P116 cells transduced with SINIL-2pr retroparticles. A-D. Flow cytometry analysis of mean EGFP expression in Jurkat cells deficient for ZAP-70 (Jurkat P116) transduced with SINIL-2pr retroparticles relative to null cells. Expression was measured at ~48 hr post-activation with 1 μM ionomycin and 10 ng/ml PMA relative to untreated counterparts. E. Cyclosporin A-sensitive induction of EGFP expression in Jurkat P116 cells transduced with SINIL-2pr. Co-stimulation of the SINIL-2pr- gene modified Jurkat P116 T-cells with 1 μM ionomycin and 10 ng/ml PMA resulted in a 3.4 ± 0.4 – fold increase in relative mean EGFP expression measured at ~48 hrs (P = 0.029). This activation-induced EGFP expression was abrogated when the cells were pretreated for ~30 minutes with 300 nM CsA.