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Table 1 Kinetic parameters used to compute the theoretical curve in FIG. 1.

From: Rotavirus NSP4114-135 peptide has no direct, specific effect on chloride transport in rabbit brush-border membrane

 

Kinetic parameter

F tests

 

V

Ki

ni

F

[df]

P

NV464-483

102 ± 8

0.34 ± 0.07

1.1 ± 0.3

0.307

[4, 34]

NS

NSP4114-135

100 ± 3

0.56 ± 0.07

1.1 ± 0.2

0.962

[4, 61]

NS

Overall fit

99 ± 3

0.43 ± 0.04

1.3 ± 0.2

0.332

[5, 101]

NS

Overall fit

102 ± 3

0.41 ± 0.04

{1}

0.637

[6, 101]

NS

Overall fit

94 ± 2

0.47 ± 0.02

{2}

1.279

[6, 101]

NS

Curve comparison

   

F'

[df ]

P

NSP4114-135 versus NV464-483

   

3.84

[3, 103]

NS

  1. Kinetic parameters (± SD) for the function v = f [I] (at constant substrate concentration = 15 mM) were estimated by applying the following equation: v = V/(1 + {([I]/Ki) ni}) where V is an apparent maximum velocity; Ki is the apparent affinity constant for the inhibitor, I (the peptide) and ni is the Hill number. (See equation 2 in [6]). Units: V = absolute uptake rates in pmoles·mg membrane protein-1·s-1 ; Ki = mM. For the F test, the degrees of freedom [df] for pure error and lack of fit, in that order, are indicated in brackets. P = NS (not significant to p < 0.01) means that, for each given curve, the data points do not differ statistically from the theoretical fit of the equation under study. For the F' test, the results were further compared by pairs where NS indicates that the two curves do not differ significantly from one another at p < 0.01. Because both peptides gave statistically indistinguishable data, the relevant data were pooled and fitted again to yield the indicated overall fit values. The symbol {} means that the ni value was fixed to an integer value before running the iteration. Further details in the text.
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Virology Journal

ISSN: 1743-422X