Effect of myristic acid inhibitors on the release of LASV particles. Vero cells were infected with LASV (strain Josiah) at a multiplicity of infection of 0.1. At 1 h post-infection, cells were either treated with 3 different myristic acid inhibitors or left untreated as indicated. A. 48 hours later cell supernatants were collected and virus yields were determined by TCID50. B. Virions of cell culture supernatants were passed through a 20% sucrose cushion by ultracentrifugation. Cell lysate and purified virions were subjected to SDS-PAGE followed by immunoblotting using antiserum specific for indicated proteins.