Figure 5

Effect of myristic acid inhibitors on the release of LASV particles. Vero cells were infected with LASV (strain Josiah) at a multiplicity of infection of 0.1. At 1 h post-infection, cells were either treated with 3 different myristic acid inhibitors or left untreated as indicated. A. 48 hours later cell supernatants were collected and virus yields were determined by TCID₅₀. B. Virions of cell culture supernatants were passed through a 20% sucrose cushion by ultracentrifugation. Cell lysate and purified virions were subjected to SDS-PAGE followed by immunoblotting using antiserum specific for indicated proteins.