Figure 4
From: Replication of a hepatitis C virus replicon clone in mouse cells
Northern Blot detection of sgJFH-1 RNA in AML12 mouse hepatocytes and NIH3T3 mouse fibroblasts. (A) Negative-strand HCV (top panel), positive-strand HCV (middle panel), and cellular GAPDH (bottom panel) was detected by Northern Blot analysis of total RNA isolated from non-transfected (NT) parental AML12 cells (lane 1) and 8 individual sgJFH-1 RNA transfected clones (lanes 2–9). In vitro transcribed positive-strand (lane 10) and negative-strand (lane 11) sgJFH-1 RNA was included as controls. (B) Northern Blot analysis for negative-strand HCV (top panel) and cellular GAPDH (bottom panel) was performed on total RNA isolated from non-transfected (NT) parental NIH3T3 cells (lane 10) and 9 randomly chosen sgJFH-1 RNA transfected clones (lanes 1–9).