APOBEC3G protein level is not altered by infection with VACV. HeLa-APOBEC3G cells were infected with VACV strain WR at an MOI of 2. Cell lysates of infected and uninfected cells were obtained 0, 24 and 48 h post infection. APOBEC3GmycHis expression was analyzed by Western blot with a mouse anti-6xHis antibody (Acris, Hiddenhausen, Germany). After stripping the blot, infection was confirmed with an anti-E3L antibody (generous gift of B. Jacobs) and equal loading with an anti-β-actin antibody (Sigma-Aldrich).