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Figure 3 | Virology Journal

Figure 3

From: Infection of human cytomegalovirus in cultured human gingival tissue

Figure 3

Expression of HCMV lytic proteins as determined by Western blot analysis. Protein samples were isolated from the cultured EpiGingival tissues that were either mock-infected (lanes 1, 5, 9, and 13) or infected with HCMV (2 × 104 PFU) (lanes 2–4, 6–8, 10–12, and 14–16) for 6 days, separated in SDS-polyacrylamide gels, and then transferred to membranes. One membrane was allowed to react with a monoclonal antibody (Anti-actin) against human actin (D) while the others were stained with the antibodies (Anti-IE1, Anti-UL44, and Anti-UL99) against HCMV IE1, UL44, or UL99, respectively (A-C). The expression of human actin was used as the internal control for the quantitation of the expression of HCMV proteins.

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