Figure 3From: Infection of human cytomegalovirus in cultured human gingival tissueExpression of HCMV lytic proteins as determined by Western blot analysis. Protein samples were isolated from the cultured EpiGingival tissues that were either mock-infected (lanes 1, 5, 9, and 13) or infected with HCMV (2 × 104 PFU) (lanes 2–4, 6–8, 10–12, and 14–16) for 6 days, separated in SDS-polyacrylamide gels, and then transferred to membranes. One membrane was allowed to react with a monoclonal antibody (Anti-actin) against human actin (D) while the others were stained with the antibodies (Anti-IE1, Anti-UL44, and Anti-UL99) against HCMV IE1, UL44, or UL99, respectively (A-C). The expression of human actin was used as the internal control for the quantitation of the expression of HCMV proteins.Back to article page