Table 1 Codon optimized sequences of mutagenesis primers

HPV33:BC

For GGCCATCCATATTTTCCCATCAAGAAGCCCAACAACAACAAATTATTGGTACCC

Rev GGGTACCAATAATTTGTTGTTGTTGGGCTTCTTGATGGGAAAATATGGATGGCC

HPV33:DE

For TTTGATGACATCGAAAACGCCAGCGCCTACGCCGCCAACGCCGGTGCTGATAATAGG

Rev CCTATTATCAGCACCGGCGTTGGCGGCGTAGGCGCTGGCGTTTTCGATGTCATCAAA

HPV33:FGa

For ATGTTTGTAAGACACCTGTTCAACAGGGCCGGCGCCTACGGCGAGAACGTTCCCGATGACCTG

Rev CAGGTCATCGGGAACGTTCTCGCCGTAGGCGCCGGCCCTGTTGAACAGGTGTCTTACAAACAT

HPV33:FGb

For ATTAAAGGTTCAGGAAGCACCGCCAACCTGGCCAGCAGCAACTACTTTCCCACTCCTAGTGG

Rev CCACTAGGAGTGGGAAAGTAGTTGCTGCTGGCCAGGTTGGCGGTGCTTCCTGAACCTTTAAT

HPV33:HI

For AATATGACTTTATGCGCCGCCATCAGCACCAGCGAGACCACCTACAAGAACAACAATTTTAAAGAATATATAAG

Rev CTTATATATTCTTTAAAATTGTTGTTCTTGTAGGTGGTCTCGCTGGTGCTGATGGCGGCGCATAAAGTCATATT

HPV16:BC

For GGCCACCCCTACTTCAGCATCAAGAACCCCACCAACGCCAAGAAGATCCTGGTGCCC

Rev GGGCACCAGGATCTTCTTGGCGTTGGTGGGGTTCTTGATGCTGAAGTAGGGGTGGCC

HPV16:DE

For ACCGGCAACAAGTACCCCGGCCAGCCCGGCGTGGACAACAGGGAGTGCATCAGCATGGAC

Rev CCTGTTGTCCACGCCGGGCTGGCCGGGGTACTTGTTGCCGGTCTCGGTGTCGTCCAG

HPV16:FGa

For ATGTTCGTGAGGCACTTCTTCAACAGGGCCGGCACCCTGGGCGAGGCCGTGCCCGACGACCTG

Rev CAGGTCGTCGGGCACGGCCTCGCCCAGGGTGCCGGCCCTGTTGAAGAAGTGCCTCACGAACAT

HPV16:FGb

For ATCAAGGGCAGCGGCACCACCGCCAGCATCCAGAGCAGCGCCTTCTTCCCCACCCCCAGC

Rev GCTGGGGGTGGGGAAGAAGGCGCTGCTCTGGATGCTGGCGGTGGTGCCGCTGCCCTTGAT

HPV16:HI

For AACATGAGCCTGTGCACCCAGGTGGCCAGCGACAGCACCTACAAGAACGAGAACTTCAAGGAGTACCTG

Rev CAGGTACTCCTTGAAGTTCTCGTTCTTGTAGGTGCTGTCGCTGGCCACCTGGGTGCACAGGCTCATGTT