Analysis of deletions found in patient 313 sera and CIMM-HCV isolates. Agarose gel electrophoresis of RT-PCR products of HCV RNA isolated from plasma and in vitro cultures. A 100 bp ladder was used (NE Biolabs). Lane 1 is 313 plasma, lane 2 is 313-i, lane 3 is 313-T1. The 269 bp fragment contains the 5'UTR. The approximately 150 bp fragments observed in 313 plasma were shown by sequencing to contain large deletions of the 5'UTR.