Analysis of in vitro replicated human hepatitis C virus (HCV) for the determination of genotypes and quasispecies

Table 1 List of CIMM-HCV isolates analyzed^a

Isolate	No. of clones sequenced	Date of transmission	Date of HCV isolation and/or RT-PCR	Days in culture
313 plasma	60	09/27/2004^b	10/10/2004
313-I	26	9/27/2004	10/18/2004	21
313-T1	26	10/10/2004^c	10/18/2004	21
238 plasma	25	08/30/2002^b	8/30/2002
238-T1	26	09/01/2002^c	2/24/2005	909
081 serum	26	03/16/2001^b	3/16/2001
081-T1	25	04/08/2001^c	2/24/2005	1441
112B-T1	27	04/08/2001^c	4/24/2005	1500
112A-T1	25	04/08/2001^c	9/11/2001	179
112AB-T1	26	04/08/2001^c	7/21/2001	127
PCLB-T1	26	04/08/2001^c	9/14/2001	182
PCLB-T4a	25	08/08/2001^c	10/12/2001	210
PCLB-T4b	26	08/08/2001^c	2/4/2005	1421
PCLB-T7	25	10/02/2001^c	10/10/2001	208

^a Samples from sera and isolates were cloned and sequenced. The dates of transmission indicate the date that HCV was added to the cells. For the T1 through T7 isolates, the exact date of transmission sometimes wasn't known, but the first date of isolation of HCV RNA from that isolate was used to obtain an approximate date.
^b Date HCV isolated from blood
^c Approximate date of transmission

ISSN: 1743-422X