Variability of CIMM-HCV samples. A) Sequence complexity of HCV samples. Shannon entropy, normalized for the number of samples and Pn variability as described by Cabot et al. (2000) and Pawlotsky et al. (1998). B) Number of nucleotide changes in the consensus sequence compared to the consensus of HCV in patient sera. C) Shannon entropy compared against the number of cell-free transfers of HCV into new cell lines. The trend line is a linear fit. D) Comparisons of Shannon entropy against categories of incubation. The error bars represent the standard deviations of the sample entropies. Days of incubation are the days that the isolate was in culture.