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Figure 2 | Virology Journal

Figure 2

From: Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro

Figure 2

Assembly of a chTCR cassette. Antibody VL (red) and VH (yellow) were linked by a flexible 15 amino acid hinge to allow correct scFv refolding and reconstitution the antigen binding site. Two thirds of the hinge sequence was attached using specifically designed PCR primers, allowing their splicing to create a scFv. To avoid creation of a sub-optimal (Gly4Ser)2 hinge, alternative glycine codons were used. To the C terminus of the scFv, extracellular and transmembrane portions of either mouse or human CD28 (etm28) are covalently attached to aid interaction between the chTCR and target and enable fluidity of the chTCR within the cell membrane. The remainder of the chTCR is as described.

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