| Â | Â | Â | Â | Tracheal Protection | Kidney Protection |
|---|
GroupA | nB | Vaccination | ChallengeC | Virus ReisolationD | Tracheal HistopathologyE | Kidney histopathology | Immunoflourscence |
|---|
| Â | Â | Â | Â | Positive | Negative | Positive | Negative | Normal | Focal | Multifocal | Diffuse | Positive | Negative | IntensityF |
|---|
A | 12 | + | + | 5 | 7 | 4 | 8 | 9 | 3 | 0 | 0 | 3 | 9 | + |
B | 5 | - | + | 5 | 0 | 5 | 0 | 0 | 3 | 4 | 0 | 5 | 0 | +++ |
C | 12 | + | - | 0 | 12 | 0 | 12 | 12 | 0 | 0 | 0 | 0 | 12 | - |
- AGroups A and C were vaccinated by IBV H120 vaccine at 1-day of age while birds in group B were kept as unvaccinated control.
- BNumber of birds used per group.
- C Four weeks post vaccination; chickens in group A and B were challenged by eye drop with 105 EID50 per bird of Egypt/F/03 while birds in group C were not challenged and kept as vaccinated unchallenged control:(-) denotes unchallenged, while (+) denotes challenged groups.
- D Virus reisolation 4 days post challenge with Egypt/F/03 in SPF ECE. Unprotected tracheal samples showed positive IBV reisolation while protected tracheal samples showed negative results.
- ETracheal histopathology 4 days post challenge with Egypt/F/03 was measured as tracheal histopathological scores. Tracheal samples showed high tracheal histopathological scores (score range: 6–8) were considered positive (unprotected). Negative (protected) tracheal scores range was 1–2.
- F Intensity of IFA: (+) denotes few cells showed positive IFA reaction, (++) many cells showed IFA reaction, (+++) diffuse IFA reaction.
