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Figure 3 | Virology Journal

Figure 3

From: Caveolin-1 interacts with the Gag precursor of murine leukaemia virus and modulates virus production

Figure 3

Co-immunoprecipitation of Cav-1. Lysates from or transfected (lane 1–3) or mock-transfected (lane 4) NIH3T3 cells were treated with rabbit anti Cav-1 followed by capture on paramagnetic protein G microbeads (μ column system, Miltenyi). Precipated proteins were separated by SDS-PAGE followed by Western Blot detection on PVDF membranes using an GFP antibody with GFP, YFP and CFP specifity. NIH3T3 lysates transfected with Lane1: GFP Plasmid; Lane 2: Gag-CFP; Lane 3: Gag-YFP Lane 4: mock; M Marker, molecular weight in kDa

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