Figure 2From: Caveolin-1 interacts with the Gag precursor of murine leukaemia virus and modulates virus productionColocalization studies using confocal microscopy. A. Caveolin-1-GFP and GagRFP fusion proteins colocalize in transiently transfected NIH 3T3 cells predominantly at the plasma membrane. NIH 3T3 cells were co-transfected with Gag-RFP and caveolin-1 GFP plasmids, fixed 46 h later and analysed by confocal microscopy. B. Caveolin-1 and GagRFP colocalization in NIH3T3 cells. NIH3T3 transfected with GagRFP plasmid were fixed 46 h after transfection and stained for immunofluorescence rabbit anti-caveolin-1antibody followed by goat anti-rabbit-Alexa 488 conjugateBack to article page