Figure 3
From: Mutational analysis of the potential catalytic residues of the VV G1L metalloproteinase
G1L undergoes proteolytic processing. BSC40 cells were transfected with 1.5 μg of plasmid DNA containing either wild type G1L or one of the eight single-site mutants. Four hours later, the transfection solution was removed and cells were infected with VV strain WR at an MOI of two. Twenty-four hours later, cells were harvested and extracts were subjected to Western blot analysis with anti-Flag antisera. The top panel demonstrates full length G1L, indicated by the arrow, and the lower panel shows the resulting cleavage products indicated by *.