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Figure 5 | Virology Journal

Figure 5

From: Involvement of intracellular free Ca2+ in enhanced release of herpes simplex virus by hydrogen peroxide

Figure 5

Effect of Ca2+ depletion on cell viability. HSV-1-infected FI cells were treated with 1 mM H2O2 from 18 to 20 h p.i. and then trypan blue-positive cells were determined. For the depletion of extracellular Ca2+ or [Ca2+]i, infected cells were pretreated with 10 mM EGTA, 50 μM BAPTA or 50 μM quin-2 for 20 min. Differences of means were analyzed with the unpaired t-test. * P < 0.05 and ** P < 0.01 vs. samples exposed to H2O2 only.

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