Figure 3

Effects of Ca²⁺ depletion on viral release. HSV-1-infected FI cells were treated with 1 mM H₂O₂ from 18 to 20 h p.i. Alternatively, infected cells were pretreated with 10 mM EGTA, 50 μM BAPTA or 50 μM quin-2 for 20 min prior to H₂O₂ treatment for 2 h. After treatment with H₂O₂, the amounts of cell-free virus (A) and cell- associated virus (B) were determined. Results were compared to those for the controls and a percentage was calculated. Data are means ± SD of three determinations. Differences of means were analyzed with the unpaired t-test. * P < 0.05, ** P < 0.01 and ** P < 0.001 vs. samples exposed to H₂O₂ only.