Small RNA-directed DNA and histone methylation. (A) Schematic representation of Sau 96 I restriction sites in ACMV DNA A promoter region and expected sizes recognized by this probe (B) Southern hybridization showing fragments of ACMV generated by Sau 96 I, arrows indicate fragments generated in vector control and protected in siRNA producing plants (C) ChIP duplex PCR analysis using ACMV DNA A promoter and Tnt-retroposon specific primers, sizes are indicated. K4, anti-dimethylated histone H3 lysine 4. K9, anti-dimethylated histone H3 lysine 9. VC, vector control.