Table 4 List of the gene-specific primers used in relative quantitative PCR analysis. Gene names and accession numbers are indicated. (*) Accession numbers for S. frugiperda correspond to Spodobase identifying numbers http://bioweb.ensam.inra.fr/spodobase/
gene name | accession number | qPCR forward primer (5'-3') | qPCR reverse primer (5'-3') |
|---|---|---|---|
HdIV | |||
rep1 | AF364055 | AACGTGGAAACTTTGTCGCC | CGTTCCTGGAGGGACTACCC |
rep2 | AF364055 | TCGGTGTGCTGATTGTGAGC | TCATGTCCCAAGTCACACGG |
rep3 | AF364055 | GCCCCTGCCATTTGAAAAAT | TCGCGAATGCAGTAGCACTG |
rep4 | AY499565 | CGGCGTGTCACAAACTGTTG | GCTTCAAGATGTTGCCCCATT |
rep5 | AY499566 | GGAAGACCGCCTGCTTATCA | CCTCCGAATAAAGGCGTCAGT |
rep6 | AY499567 | AAGGCCAGAAGAAGATCGCC | AGAGGCATGAGCCAGTCCC |
rep7 | AY499568 | TCGTATCGTTCCACCGGGTA | CAGCCAGATGGTGGAAGCTC |
rep8 | AY499569 | GTTTTGCCCCAATGGTGATG | TGCCACAGTTTTGCTCGAAC |
rep11 | AY501383 | same as rep6 gene | same as rep6 gene |
rep12 | AF479654 | GGGTCGCAATGAAGGTGCTA | CTGGCGAGTGTGTTTGCAAT |
H. didymator | |||
18S RNA | AY433942 | CATCGTGGTGCTCTTCATTGA | CAAAGTAAACGTACCGGCCC |
S. frugiperda | |||
E2 ubiquitin ligase | SF9L03548 (*) | ACTTGTGGCCCGCATACACT | GGATCGGCACAATAAATGGG |
RNA polymerase II | SF9L00930 (*) | TGCCATCGGGAAAATGAAAT | TTCTCTGCACCTTATTGGGTCTC |