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Table 2 List of the gene-specific oligonucleotide probes used in Southern-blot. Hybridization temperature is indicated next to the primer

From: Members of the Hyposoter didymator Ichnovirus repeat element gene family are differentially expressed in Spodoptera frugiperda

Gene

oligonucleotide (5'-3')

Pre-hybridization & hybridization temperature

rep4

GATGTTGCCCCATTTCTAGAACCGCAACAG

48°C

rep5

AGGGGCCCCACGCGGTAGACGAAACCCACG

54°C

rep6 &11

GCCCGCGGAACGTGAAGGTGTCCACCGGGT

50°C

rep7

CCTGCGAAATTTCTTGATACACCACAGCCT

47°C

rep8

TTCTCGTTGCAGCCCGTGACAGGCGCGAGC

53°C

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Virology Journal

ISSN: 1743-422X