Figure 3
Expression profiles and gene copy number of the 10 rep genes identified in HdIV by relative quantitative PCR. A. Transcript levels in 2nd instar S. frugiperda parasitized larva, over 1-h to 24-h time course study. B. Relative gene copy numbers in HdIV genome. C. Transcript levels in H. didymator adult female and male wasps. D. Transcript levels in different tissues of last instar S. frugiperda larvae 24 hours after injection of HdIV (H: Hemocyte; FB: Fat Body; Ep: Cuticular Epithelium; SN: Nervous System (Head); TD: Digestive Track). Data are means ± SE of starting quantity of fluorescence (N0 value) for 6–9 measurements. For A, C and D, data are normalized to housekeeping genes RNA polymerase II and E2 ubiquitin ligase. For details, address to Methods chapter.