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Table 4 Efficiency with which wild-type HSV-1 and ICP0- viruses reactivate from latently infected trigeminal ganglia.

From: ICP0 antagonizes Stat 1-dependent repression of herpes simplex virus: implications for the regulation of viral latency

   ACUTE INFECTION REACTIVATION  
Mouse strain Virusa Inoculumb Survivalc Viral spread to L7 cellsd Virus in TG on Day 14e Viral genomes per TGf
Strain 129 KOS 2 × 105 100% (n = 15) 11/16 14/16 3.0 × 105
Strain 129 0--GFP 2 × 105 100% (n = 15) 0/16 0/16 1.3 × 105 *
ifngr -/- 0--GFP 2 × 105 100% (n = 25) 0/38 0/38 1.2 × 105 *
ifnar -/- 0--GFP 2 × 105 100% (n = 21) 0/16 2/16 3.5 × 105
rag2 -/- ifnar -/- 0--GFP 2 × 104 0% (n = 11) NDh ND ND
ifnar -/- ifngr -/- 0--GFP 2 × 104 21% (n = 14) 0/6 0/6 ND
stat1 -/- 0--GFP 2 × 104 50% (n = 50) 0/22 2/22 1.8 × 105
  1. a Virus used to inoculate mice.
  2. bDose of virus in 4 μl viral inoculum applied to each eye (i.e., pfu per eye).
  3. cThe percentage of mice that survived until Day 38 p.i., when mice were first sacrificed for experiments.
  4. dFrequency of KOS or 0--GFP reactivation in latently infected trigeminal ganglia, as determined by the development of cytopathic effect in monolayers of L7 cells co-cultured with ganglion explants.
  5. eFrequency of KOS or 0--GFP reactivation from latently infected trigeminal ganglia, as determined by the presence of infectious virus in trigeminal ganglion homogenates on day 14 post explant.
  6. fThe average number of viral genomes per TG, as determined by competitive PCR analysis of TG DNA harvested from each treatment group of mice (per the results presented in Figure 8).
  7. hNot determined because too few mice survived the acute infection.
  8. * p < 0.05 that 0--GFP genome loads in latently infected TG were equivalent to wild-type levels of viral genomes in KOS latently-infected TG, based on a two-way t-test.