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Table 3 Abundance of LAT+ versus HSV antigen+ neurons in trigeminal ganglia infected with HSV-1 strain KOS.

From: ICP0 antagonizes Stat 1-dependent repression of herpes simplex virus: implications for the regulation of viral latency

  Day 3 Day 5.5
Micea LAT+ neuronsb HSV Ag+ neuronsc LAT+ neurons HSV Ag+ neurons
wild-type 0.02 – 0.04% (12)d 7 – 15% (4399) 0.1 – 0.2% (45) 9 – 18% (5300)
rag2 -/- 0.02 – 0.04% (12) 10 – 20% (6122) 0.1 – 0.3% (82) 18 – 36% (10897)
stat1 -/- 0.03 – 0.06% (18) 10 – 20% (5978) 0.1 – 0.2% (48) > 20% (TNTC)e
rag2 -/- stat1 -/- 0.02 – 0.04% (11) 8 – 17% (5005) 0.1 – 0.2% (59) > 20% (TNTC)
  1. aMice were inoculated with 2 × 105 pfu per eye of wild-type HSV-1 strain KOS, and were sacrificed 72 hours (Day 3) or 132 hours (Day 5.5) after inoculation to measure the relative abundance of trigeminal ganglion neurons that expressed HSV-1 LAT RNA versus viral antigens.
  2. bNeurons in 7 μM sections of KOS-infected trigeminal ganglia (TG) that hybridized with a latency-associated transcript (LAT)-specific riboprobe and which were HSV antigen-negative, as determined under a fluorescent microscope.
  3. cNeurons in 7 μM sections of KOS-infected trigeminal ganglia (TG) that were labeled by rabbit anti-HSV antibody and which were LAT-negative, as determined under a fluorescent microscope.
  4. dThe estimated percent of TG neurons that were LAT+, or HSV antigen+, is based on the total number of neurons counted (number shown in parentheses) in a total of 144 TG sections derived from 6 independent TG (i.e., 24 sections per TG were analyzed). It was estimated that this number of sections contained at least 30,000 neurons but not more than 60,000 neurons. The reported range of percentages of LAT+ or HSV antigen+ neurons was estimated by dividing the number of counted neurons by these lower or upper limits (30,000 or 60,000), and by multiplying times 100 to convert to a percentage.
  5. eTNTC, too numerous to count.