ICP0 antagonizes Stat 1-dependent repression of herpes simplex virus: implications for the regulation of viral latency

Table 2 Effect of interferon receptors versus Stat 1 on HSV-1 shedding and the survival of infected mice.

		Viral titers per eye on day 3 p.i.^c	Survival
Virus^a	Mouse strain^b		Frequency^d	Duration (days)^e
	wild-type	1.8 ± 0.3	100%	> 60
	rag2 ^-/-	1.5 ± 0.4	0%	18 ± 0.5
	ifngr ^-/-	1.8 ± 0.5	100%	> 60
	ifnar ^-/-	2.8 ± 0.3	50%	11 ± 1^‡
KOS-GFP (ICP0⁺)	ifnar ^-/- ifngr ^-/-	3.6 ± 0.1*	0%	5.2 ± 0.1^‡
	stat1 ^-/-	4.3 ± 0.1*	0%	8.1 ± 0.3^‡
	rag2 ^-/- stat1 ^-/-	4.0 ± 0.2*	0%	8.6 ± 0.2^‡
	rag2 ^-/- ifnar ^-/-	4.2 ± 0.2*	0%	7.9 ± 0.2^‡
	wild-type	1.1 ± 0.6	center100%	> 60
	rag2 ^-/-	0.7 ± 0.5	100%	> 60
	ifngr ^-/-	1.4 ± 0.4	100%	> 60
	ifnar ^-/-	2.2 ± 0.1	100%	> 60
0^--GFP (ICP0^-)	ifnar ^-/- ifngr ^-/-	3.4 ± 0.3*	16%	11 ± 1^‡
	stat1 ^-/-	4.4 ± 0.1*	50%	11 ± 1^‡
	rag2 ^-/- stat1 ^-/-	3.4 ± 0.2*	0%	11 ± 1^‡
	rag2 ^-/- ifnar ^-/-	3.8 ± 0.2*	0%	10 ± 1^‡

^a Mice were inoculated with 2 × 10⁵ pfu per eye of HSV-1 strain KOS-GFP or 0^--GFP.
^b The relationship of the genotype and phenotype of these mice is defined in Table 1.
^c The mean ± standard error of the mean of the logarithm of infectious viral titers recovered from the ocular tear film of mice at day 3 p.i. (n = 6 per group).
^d The percentage of mice that survived until day 60 p.i.
^e The mean ± standard error of the mean duration of survival of those mice that survived for less than 60 days after inoculation with HSV-1. Groups of mice in which no deaths were recorded were sacrificed on day 60 p.i., and their duration of survival is indicated as "> 60."
* p < 0.05 that viral titers on day 3 p.i. were equivalent to those recovered from wild-type mice infected with the same virus, based on one-way ANOVA and Tukey's post hoc t-test.
^‡ p < 0.05 that the duration of survival was equivalent to the duration of survival of rag2^-/- (lymphocyte-deficient) mice infected with the same virus, based on one-way ANOVA and Tukey's post hoc t-test.

ISSN: 1743-422X