Table 1A Probes for housekeeping genes
Oligo Name | Unigene | nt | Transcript Length (nt) | Distance from 3' (nt) | Tm (°C) | GC (%) | Design | Sense Probe Sequence (5' to 3') |
|---|---|---|---|---|---|---|---|---|
RPL37A | Hs.433701 | 70 | 1059 | 607 | 78.3 | 50.0 | CS | AGGCCTTCCCGAGAAAGTGCTTAGCCTTGTTGATGATCCAAGGAACCACATAGAGAACCAAGACGAGTGC |
KIAA0220 | Hs.110613 | 70 | 1121 | 416 | 82.4 | 60.0 | CS | TGGAACCATCATCACCCGAACCCAAGAGGCGGAGGGTCGGTGACGTGGAACCGTCACGCAAACCCAAGAG |
CLU | Hs.75106 | 70 | 1676 | 903 | 82.4 | 60.0 | CS | TTTCCCAAGTCCCGCATCGTCCGCAGCTTGATGCCCTTCTCTCCGTACGAGCCCCTGAACTTCCACGCCA |
MTA2 | Hs.118786 | 70 | 1929 | 154 | 78.8 | 51.4 | CS | GCAAGAAGTTACGACACGTACACAACGACAGAACAACAGAGAAGACCCCGAAGACCACTAGCACGACCGT |
384D8-2.2 | Hs.356523 | 71 | 2281 | 334 | 80.6 | 52.2 | CS | CGAAGGAAAGTGGAGCTCTTCATCGCCACCTCCCAGAAGTTTATCCAGGAGACAGAGCTGAGCCAGCGCA |
FTL | Hs.118786 | 70 | 1929 | 154 | 78.8 | 51.4 | CS | CTCTCTCTTTCAGGCCTCAACAGGCACTGTATTCATTGCCAATGTTCCAAATTATCAAATTCAAGTGAAT |
PSMD2 | Hs.74619 | 70 | 2828 | 122 | 75.9 | 44.3 | CS | TATCTTCGGAAGAACCCCAATTATGATCTCTAAGTGACCACCAGGGGCTCTGAACTGTAGCTGATGTTAT |
PSMB3 | Hs.82793 | 70 | 692 | 42 | 79.4 | 52.8 | CS | ATCATCGAGAAGGACAAAATCACCACCAGGACACTGAAGGCCCGAATGGACTAACCCTGTTCCCAGAGCC |
T CFL1 | Hs.2430 | 70 | 1324 | 153 | 76.5 | 46.2 | CS | CCCCGAGCCTTGCGCCAGAAAATTGTCATTAAATGAAGAGATGTCTAGTCCTCAGAAACTTCTTTCCTGC |
H3F3A | Hs.181307 | 70 | 1305 | 20 | 73.6 | 38.5 | CS | GAGTTGTCCTACATGCAAGTACATGTTTTTAATGTTGTCTGTCTTCTGTGCTGTTCCTGTAAGTTTGCTA |
PTDSS1 | Hs.77329 | 70 | 2504 | 242 | 77.7 | 48.6 | CS | GTAGCTGCCTGCATAGGAGCCTCGCTTCCGATTATTCCCTTCCCAATATTATTCATCCAGACTTAGCCAC |
KARS | Hs.3100 | 70 | 1997 | 142 | 73.6 | 38.6 | CS | GCAACCACTGATACACTGGAAAGCACAACAGTTGGCACTTCTGTCTAGAAAATAATAATTGCAAGTTGTA |
AAMP | Hs.83347 | 70 | 1762 | 622 | 81.2 | 57.1 | CS | ACCTTGGCCATCTATGACCTGGCTACGCAGACTCTTAGGCATCAGTGTCAGCACCAGTCGGGCATCGTGC |
β-actinsense | Hs.288061 | 68 | 1841 | 431 | 92.7 | 50.0 | PE | TTAAAAACTGGAACGGTGAAGGTGACAGCAGTCGGTTGGAGCGAGCATCCCCCAAAGTTCACAATGTG |
β-actin.70 mer | Hs.288061 | 71 | 1841 | 700 | 96.0 | 56.3 | PE | CCTGGCACCCAGCACAATGAAGATCAAGATCATTGCTCCTCCTGAGCGCAAGTACTCCGTGTGGATCGGCG |
c-yes.70 mer | Hs.194148 | 70 | 4343 | 1249 | 96.7 | 62.9 | PE | CTCGGCTCACTGCAAGCTCTGCCTCCCAGGTTCACACCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGG |
c-yes.2 | Hs.194148 | 70 | 539 | 73.6 | 52.2 | CS | CATGCAAGTTGGCAGTGGTTCTGGTACTAAAAATTGTGGTTGTTTTTTCTGTTTACGTAACCTGCTTAGT | |
MHCI.70 mer | Hs.379218 | 70 | 2290 | 600 | 92.7 | 55.7 | PE | CTCAGATAGAAAAGGAGGGAGCTACTCTCAGGCTGCAAGCGGCAACAGTGCCCAGGGCTCTGATGTGTCT |
HMBS.70 mer | Hs.82609 | 70 | 1536 | 1300 | 97.7 | 57.0 | PE | ACGGCAATGCGGCTGCAACGGCGGAAGAAAACAGCCCAAAGATGAGAGTGATTCGCGTGGGTACCCGCAA |
HMBS.2 | Hs.82609 | 70 | 164 | 82.4 | 52.2 | CS | TGCTGTCCAGTGCCTACATCCCGGGCCTCAGTGCCCCATTCTCACTGCTATCTGGGGAGTGATTACCCCG | |
EF1.70 mer | Hs.181165 | 71 | 1833 | 500 | 91.8 | 50.7 | PE | GGCAAGCCCATGTGTGTTGAGAGCTTCTCAGACTATCCACCTTTGGGTCGCTTTGCTGTTCGTGATATGAG |