Figure 4

Effect of induction of lytic EBV infection on Zta and EBV gene transcription. (A) Western blot showing protein transcription levels of Zta in IgG cross-linking induced (+) and non-treated (-) Akata cells. (B) Transcription of EBV genes was quantified by microarray analysis in Akata cells upon induction of lytic infection. Treated and non-treated cells were harvested at different times after induction of lytic infection by BCR cross-linking. Competitive hybridization of labeled samples from treated cells was performed against non-treated cells. Shown are mean values of two dye-swap experiments. (C) EBV gene transcription was quantified during induction of lytic EBV infection in Akata cells by BCR cross-linking. For each time point, treated and non-treated cells were harvested and subjected to qPCR. Each point represents the difference between induced and non-induced cells, normalized to the HMBS housekeeping gene. Results are from at least two biological replicates and are given as: ΔΔC_T = (C_T(EBV gene)-C_T(HMBS)}_treated-{C_T(EBV gene)-C_T(HMBS))_{not treated.}