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Figure 4 | Virology Journal

Figure 4

From: The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein GP-C

Figure 4

Glycosidase sensitivity of cell surface transported Lassa virus glycoprotein . At 48 h post-transfection, Vero cells expressing wild-type Lassa virus glycoprotein were surface biotinylated and immunoprecipitated as described under Fig. 3. Precipitated samples were either left untreated (lane 1), digested with Endo H (lane 2) or PNGase F (lane 3). The samples were separated by SDS-PAGE with subsequent immunoblotting. Biotinylated proteins were detected using streptavidin coupled to horse radish peroxidase and chemiluminescence.

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Virology Journal

ISSN: 1743-422X