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Figure 3 | Virology Journal

Figure 3

From: The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein GP-C

Figure 3

Cell surface transport of GP-C N-glycosylation mutants . Vero cells were transfected with GP-1 (A) and GP-2 (B) N-glycosylation mutants of Lassa virus glycoprotein. At 24 h post-transfection, cells were surface labelled with biotin. Following cell lysis and immunoprecipitation using α-GP-2, samples were subjected to SDS-PAGE and blotted to nitrocellulose. Surface biotin-labelled proteins were visualized with streptavidin-peroxidase and chemiluminescence.

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