Figure 2
From: Expression and processing of the Hepatitis E virus ORF1 nonstructural polyprotein
Expression and processing of the ORF1 polyprotein. T. ni cells infected with the vORF1 recombinant virus were harvested at various times post-infection and the lysates subjected to SDS-PAGE and western blotting with anti-His (A) or anti-FLAG (B) antibodies. Lanes 1 to 5 show results at 24, 36, 48, 60 and 72 hr post-infection; lane 6 shows the result for wild type AcMNPV infection after 48 hr. Panel C shows the 48 hr lysate probed with anti-MeT antibodies. In (B) the upper and lower panels show results from 7.5% and 12% SDS-polyacrylamide gels. The estimated fragment sizes are shown based on molecular size markers run on each gel (not shown).