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Table 1 Summary of results for HRSV F cysteine mutants. Processing is defined as relative amounts of F0, F1, and F2, and is described as being equivalent to wild-type HRSV F protein (complete) or reduced. Cell surface and total expression were measured by ELISA under permeabilizing (total F protein) or non-permeabilizing (cell surface F protein) conditions using palivizumab as described in methods and reported as percent relative to wild-type HRSV F protein. Reactivity with neutralizing mAbs (palivizumab, Mab19, 47F, and 101F) as determined by flow cytometry is shown and reported as percent relative to wild-type HRSV F protein. Cell fusion activity is reported as luciferase activity measured at 32°C, 37°C, and 39.5°C as described in [44]. All values are expressed as % relative to wild-type at the respective temperatures.

From: Contribution of cysteine residues in the extracellular domain of the F protein of human respiratory syncytial virus to its function

 

Protein Processing

ELISA

Cell surface expression (Flow cytometry)

Cell fusion (% of WT)

  

Cell surface protein (Non-permeabilized)

Total protein (permeabilized)

Palivizumab

47F

101F

mAb19

32°C

37°C

39.5°C

Wild-type

complete

100

100

100

100

100

100

100%

100%

100%

C37S

minimal

0.00

51

7

3

2

4

8%

8%

12%

C69S

reduced

25

72

22

19

21

20

10%

12%

12%

C212S

complete

117

103

37

43

46

39

52%

44%

34%

C313S

reduced

3

44

23

11

4

1

6%

5%

5%

C322S

minimal

0

35

5

1

3.5

0

7%

5%

6.5%

C333S

minimal

0

41

8

3

3

0.5

12%

8.5%

10%

C343S

minimal

0

22

4

0

1.5

0

14%

17%

19%

C358S

minimal

0

17

3

0

2

0

7%

4%

6%

C367S

minimal

0

8

8

3

9

1.5

10%

8%

9%

C382S

complete

103

90

86

102

96

88

105%

91%

100%

C393S

reduced

8

42

13

10

13

7

14%

10%

12%

C416S

minimal

0

43

4

3

5

0

5.5%

4%

4%

C422S

complete

141

132

90

93

81

81

140%

122%

146%

C439S

minimal

0.4

26

7

4

4

1

50%

29%

30%