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Table 1 Summary of results for HRSV F cysteine mutants. Processing is defined as relative amounts of F0, F1, and F2, and is described as being equivalent to wild-type HRSV F protein (complete) or reduced. Cell surface and total expression were measured by ELISA under permeabilizing (total F protein) or non-permeabilizing (cell surface F protein) conditions using palivizumab as described in methods and reported as percent relative to wild-type HRSV F protein. Reactivity with neutralizing mAbs (palivizumab, Mab19, 47F, and 101F) as determined by flow cytometry is shown and reported as percent relative to wild-type HRSV F protein. Cell fusion activity is reported as luciferase activity measured at 32°C, 37°C, and 39.5°C as described in [44]. All values are expressed as % relative to wild-type at the respective temperatures.

From: Contribution of cysteine residues in the extracellular domain of the F protein of human respiratory syncytial virus to its function

  Protein Processing ELISA Cell surface expression (Flow cytometry) Cell fusion (% of WT)
   Cell surface protein (Non-permeabilized) Total protein (permeabilized) Palivizumab 47F 101F mAb19 32°C 37°C 39.5°C
Wild-type complete 100 100 100 100 100 100 100% 100% 100%
C37S minimal 0.00 51 7 3 2 4 8% 8% 12%
C69S reduced 25 72 22 19 21 20 10% 12% 12%
C212S complete 117 103 37 43 46 39 52% 44% 34%
C313S reduced 3 44 23 11 4 1 6% 5% 5%
C322S minimal 0 35 5 1 3.5 0 7% 5% 6.5%
C333S minimal 0 41 8 3 3 0.5 12% 8.5% 10%
C343S minimal 0 22 4 0 1.5 0 14% 17% 19%
C358S minimal 0 17 3 0 2 0 7% 4% 6%
C367S minimal 0 8 8 3 9 1.5 10% 8% 9%
C382S complete 103 90 86 102 96 88 105% 91% 100%
C393S reduced 8 42 13 10 13 7 14% 10% 12%
C416S minimal 0 43 4 3 5 0 5.5% 4% 4%
C422S complete 141 132 90 93 81 81 140% 122% 146%
C439S minimal 0.4 26 7 4 4 1 50% 29% 30%