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Figure 2 | Virology Journal

Figure 2

From: VPg of murine norovirus binds translation initiation factors in infected cells

Figure 2

MNV-1 GST-VPg binds eIF3, phospho-eIF4GI, eIF4E, and S6 ribosomal protein by pull-down assay. GST and MNV-1 GST-VPg were expressed in bacteria and purified as described in the text. A) Purified GST and GST-VPg, analyzed by 10% SDS-PAGE and stained with Coomassie blue. Proteins in the pull-down eluates were subjected to SDS-PAGE, and then immunoblot probed with (B) anti-eIF3, (C) anti-phospho-eIF4GI, (D) anti-eIF4E, and (E) anti-S6 ribosomal protein. Blots were developed with ECL chemiluminescent substrate. The asterisk in B indicates a protein identified as eIF4GI.

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