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Figure 2 | Virology Journal

Figure 2

From: Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia

Figure 2

Heparinase III treatment efficiently removes heparan sulfate from human tracheal epithelial (HTE) cell membranes. Cells were incubated with a monoclonal mouse anti-heparan sulfate (10E4 epitope) antibody followed by a Cy3 conjugated donkey anti-mouse IgG. A DAPI nuclear counterstain was applied. Treatment of HTE cells with 10 mIU and 20 mIU of heparinase III reduced membrane HSPG staining to levels seen in cells not treated with primary anti-heparan antibody.

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Virology Journal

ISSN: 1743-422X