Figure 3
Agarose electrophoretic profiles of amplification products from DNA polymerase targeted-degenerate PCR on quail and turkey isolates of avian poxvirus using the consensus lower primer with HV upper primer (A), and the poxvirus specific primer (B). Lanes are designated Q, T and N for quail virus, turkey virus and no virus infected chicken chorioallantoic membrane, respectively, + indicates the positive control (CCV DNA) and – indicates a negative water control, 1 Kb = 1 Kb ladder (Invitrogen), lower case letters indicate extraction protocols with no designation being a total DNA extraction from the tissue, p indicating pelleted sample (the virus was filtered through a 0.45 μm filter and pelleted at 20,000 × g before DNA extraction) and d indicating DNase treament (the pelleted sample was resuspended and DNase treated before DNA extraction). The > indicates product that was sequenced.