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Figure 2 | Virology Journal

Figure 2

From: A broadly applicable method to characterize large DNA viruses and adenoviruses based on the DNA polymerase gene

Figure 2

Agarose electrophoretic profiles of amplification products from DNA polymerase targeted-degenerate PCR from avian adenovirus samples (A], catfish herpesvirus samples (B), fish iridovirus samples (C) and Avian Poxvirus samples (D). The > indicates bands that were evaluated by sequencing. A-chicken adenovirus isolates CELO-lane 1, case 162-lane 2, case 1422b-lane 3 using adenovirus upper and consensus lower primers. The products of interest were 1200 bp. B-lanes designated CCV and BCV represented the type specimen (Auburn clone A) and the blue catfish isolates respectively produced 465 bp bands using HV and cons lower primers. C-Largemouth bass ranavirus type specimen-LBV, and lymphocystis disease virus case (infected fin tissue) LDV produced 695 bp and 662 bp bands, respectively, using HV and cons lower primers. D Avian Poxvirus from Quail (QPV) and turkey (TPV) produce multiple bands using the HV and Cons lower primers on DNA extracts from infected chicken chorioallantoic membranes.

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Virology Journal

ISSN: 1743-422X