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Figure 4 | Virology Journal

Figure 4

From: Inhibition of histone deacetylation in 293GPG packaging cell line improves the production of self-inactivating MLV-derived retroviral vectors

Figure 4

Titer of SINCMV retroviral producers treated with TsA and model depicting mechanism of transcriptional suppression in the SINCMV design. A. Treatment of SINCMV retroviral producer cells with the histone deacetylase inhibitor TsA for 48 hr resulted in a maximal 15.5 ± 1.3-fold increase in titer (P = 0.008) that was obtained with 3 μM TsA. B. Interferences between strong elements in the internal CMVIE enhancer-promoter and the upstream CMV promoter in the 5'LTR lead to the recruitment of histone deacetylases (HDACs) which trigger an inactive chromatin conformation at the promoter sites leading to transcriptional suppression of the retroviral RNA.

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