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Figure 2 | Virology Journal

Figure 2

From: Inhibition of histone deacetylation in 293GPG packaging cell line improves the production of self-inactivating MLV-derived retroviral vectors

Figure 2

Titer of SINCMV retroviral producers treated with sodium butyrate and transduction of A549 cells with retrovirus from butyrate treated SINCMV producer cells. A. Treatment of control retroviral producer cells with the histone deacetylase inhibitor sodium butyrate for 48 hr resulted in a modest 1.6 ± 0.4-fold increase in titer (P = 0.299). B. Treatment of SINCMV retroviral producer cells with sodium butyrate for 48 hr resulted in a maximal 106.3 ± 4.6-fold increase in titer (P = 0.002) that was obtained with 20 mM butyrate. C. A549 lung carcinoma cells were transduced with same volume of retroviral supernatant that was collected from control-untreated SINCMV producers (a), producers treated with 10 mM sodium butyrate (b), and 20 mM sodium butyrate (c). % EGFP positive cells for each sample and mean EGFP reporter expression in the gated population (MnX) indicate a marked increase in gene transfer into target cells with supernatant from butyrate treated producers.

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