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Table 1 Intra assay variability of the newly developed qPCR assay

From: Application of real-time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia

 

DNA (copies DNA per mL) in experiment

Sample ID

Experiment 1

Experiment 2

Experiment 3

Average

SD

CV (%)

MVA 300

2.6 × 106

2.5 × 106

1.6 × 106

2.3 × 106

0.104

1.60

MVA 307

1.9 × 109

4.5 × 109

1.7 × 109

2.7 × 109

0.191

2.00

MVA 313

1.2 × 108

1.2 × 108

1.2 × 108

1.2 × 108

0.004

0.50

MVA 315

2.5 × 106

2.2 × 106

1.9 × 106

2.2 × 106

0.046

0.70

T4040

4.7 × 103

4.9 × 103

6.4 × 103

5.4 × 103

0.060

1.60

Negative control

not detected

not detected

not detected

   
  1. The six samples represented in the table include five positive samples (MVA 300, MVA 307, MVA 313, MVA 315) and one negative sample (negative for HBsAg). The samples were tested three times in the same assay.
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Virology Journal

ISSN: 1743-422X