Figure 1

A-MLV binds preferentially to large rafts. A) NIH3T3 cells were incubated with GagYFP A-MLV (green) for 3 hours, fixed, and GM1 was stained with fluorescently labeled CTX (red). B) NIH3T3 cells were incubated with GagYFP A-MLV (green) for 3 hours and fixed. The cells were permeabilized with Triton X-100 and cav-1 was stained (red). C) NIH3T3 cells stably expressing cav-1 mRed fusion protein (red) were incubated with GagYFP A-MLV (green) for 3 hours and fixed. Clusters of viral particles as those found bound to large rafts in A are labelled with arrows. All pictures were taken using confocal microscopy.