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Table 2 Relative expression of MGMT and eGFP

From: Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI

Vector

Relative MGMT Expression

Relative eGFP Expression

MGMT

1.00 ± 0.14

NA

eGFP

NA

1.00 ± 0.33

MGMT-2A-eGFP

0.87 ± 0.14

0.41 ± 0.14

MGMT-IRES-eGFP

0.80 ± 0.15

0.10 ± 0.02

HOXB4-2A-MGMT-IRES-eGFP

0.45 ± 0.13

0.06 ± 0.02

MGMT-2A-HOXB4-IRES-eGFP

0.16 ± 0.04

0.04 ± 0.01

  1. NA-Not applicable
  2. Column 1: MGMT activity per average copy number is calculated relative to the expression in monocistronic vector. Both bicistronic vectors are equally good for MGMT expression (as opposed to eGFP expression). Tricistronic vectors are less efficient than mono and bicistronic vectors for MGMT expression, but downstream sequences in the tricistronic vectors also affect MGMT expression.
  3. Column 2: EGFP expression per copy number expressed relative to monocistronic eGFP vector. From this it can be seen that bicistronic 2A vector is much better than IRES based vector for eGFP expression. EGFP consistently expressed poorly when placed downstream of IRES in either bi or tricistronic vectors. All the values are expressed as mean ± SEM of 4 to 6 independent observations.
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Virology Journal

ISSN: 1743-422X