Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI

Table 1 Relative vector titers as measured by HIV-1 p24 gag protein.

We transiently transfected each one of the above mentioned transfer vector plasmids with pCMVΔR8.91 and pMD.G into 293T cells as described in methods. Vector supernatants were harvested 72 h post transfection and concentrated by ultracentrifugation. HIV-1 gag protein (p24) was estimated in the concentrated vector preparations by ELISA. Values are expressed as nanograms of p24 (mean ± SD) from 4 independent experiments. Statistical analysis was performed using analysis of variance and Tukey's studentized range test. No statistically significant difference in titer (p24) was observed between the vectors tested.

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