Figure 8From: Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOIImmunocytochemistry demonstrating expression of HOXB4 in transduced K562 and Hela cells. Immunocytochemistry was performed with rat anti HOXB4 as described in methods. Immunocytochemical detection of HOXB4 shows clear nuclear localization. A and C are K562 cells transduced with HOXB4-2A-MGMT-IRES-eGFP and MGMT-2A-HOXB4-IRES-eGFP respectively. B and D are Hela cells transduced with HOXB4-2A-MGMT-IRES-eGFP and MGMT-2A-HOXB4-IRES-eGFP respectively.Back to article page