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Figure 7 | Virology Journal

Figure 7

From: Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI

Figure 7

Comparison of IRES-mediated third gene expression in tricistronic vectors. To compare the levels of third gene product expressed downstream of IRES, we transduced K562 cells with tricistronic lentiviral vectors with eGFP placed as the third transgene as shown in Figure 1. K562 cells (5 × 104) were transduced once with viral particles in the range of 5, 10, 20, 50, 100 and 200 ng of p24. Seven days after transduction, cells were analyzed by flow cytometry for expression of eGFP. Untransduced K562 cells were used as control. The percentage of eGFP positive cells and MFI are given in each histogram for eGFP, HOXB4-2A-MGMT-IRES-eGFP and MGMT-2A-HOXB4-IRES-eGFP transduced cells.

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Virology Journal

ISSN: 1743-422X