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Figure 6 | Virology Journal

Figure 6

From: Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI

Figure 6

Immunocytochemistry demonstrating MGMT expression in K562 and Hela cells. Immunocytochemistry was performed with rabbit polyclonal anti-human MGMT antisera as described in methods. Immunocytochemical detection of MGMT shows clear nuclear localization. A, C, E are K562 cells transduced with MGMT-2A-eGFP, HOXB4-2A-MGMT-IRES-eGFP and MGMT-2A-HOXB4-IRES-eGFP respectively. B, D, F are Hela cells transduced with MGMT-2A-eGFP, HOXB4-2A-MGMT-IRES-eGFP and MGMT-2A-HOXB4-IRES-eGFP respectively.

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