Figure 6From: Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOIImmunocytochemistry demonstrating MGMT expression in K562 and Hela cells. Immunocytochemistry was performed with rabbit polyclonal anti-human MGMT antisera as described in methods. Immunocytochemical detection of MGMT shows clear nuclear localization. A, C, E are K562 cells transduced with MGMT-2A-eGFP, HOXB4-2A-MGMT-IRES-eGFP and MGMT-2A-HOXB4-IRES-eGFP respectively. B, D, F are Hela cells transduced with MGMT-2A-eGFP, HOXB4-2A-MGMT-IRES-eGFP and MGMT-2A-HOXB4-IRES-eGFP respectively.Back to article page