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Figure 5 | Virology Journal

Figure 5

From: Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI

Figure 5

(A). Western blot analysis of β-actin, eGFP and MGMT expression in transduced K562 cells. Lanes a. eGFP, b. MGMT, c. MGMT-2A-eGFP, d. MGMT-IRES-eGFP, e. HOXB4-2A-MGMT-IRES-eGFP, f. MGMT-2A-HOXB4-IRES-eGFP. Mean copy number, MGMT activity, percentage of eGFP positive cells and MFI of the given samples are indicated in the table. (B). Northern blot analysis of vector-derived transcripts in transduced K562 cells. Lanes 1. eGFP, 2. MGMT, 3. MGMT-2A-eGFP, 4. MGMT-IRES-eGFP, 5. HOXB4-2A-MGMT-IRES-eGFP, 6. MGMT-2A-HOXB4-IRES-eGFP.

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Virology Journal

ISSN: 1743-422X