Skip to main content
Figure 4 | Virology Journal

Figure 4

From: Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI

Figure 4

(A) EGFP expression (MFI) in K562 cells calculated per copy number from the flow cytometry data. Samples were selected among the cells having close to an average copy number of ~1. (a) eGFP, (c) MGMT-2A-eGFP, (d) MGMT-IRES-eGFP, (e) HOXB4-2A-MGMT-IRES-eGFP, (f) MGMT-2A-HOXB4-IRES-eGFP. (B). MGMT expression measured as biochemical activity in K562 cells following lentiviral transduction. Activity is presented as fmol/mg protein/transgene copy number. (b) MGMT, (c) MGMT-2A-eGFP, (d) MGMT-IRES-eGFP, (e) HOXB4-2A-MGMT-IRES-eGFP, (f) MGMT-2A-HOXB4-IRES-eGFP. All the values are expressed as mean ± SEM of 4 to 6 independent observations.

Back to article page

Virology Journal

ISSN: 1743-422X