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Figure 3 | Virology Journal

Figure 3

From: Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI

Figure 3

Analysis of average transgene copy number by real-time quantitative PCR. To compare the average transgene copies among the transduced K562 cells real time Q-PCR analysis was carried out using primers specific for sequences located within WPRE region of the vector.(a) eGFP, (b) MGMT, (c) MGMT-2A-eGFP, (d) MGMT-IRES-eGFP, (e) HOXB4-2A-MGMT-IRES-eGFP, (f) MGMT-2A-HOXB4-IRES-eGFP. Values are expressed as mean ± SEM of 4 to 6 independent observations.

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Virology Journal

ISSN: 1743-422X