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Figure 1 | Virology Journal

Figure 1

From: Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI

Figure 1

Schematic diagram of HIV-1 based lentiviral vectors. Monocistronic vectors: (a) eGFP, (b) MGMT, Bicistronic vectors: (c) MGMT-2A-eGFP, (d) MGMT-IRES-eGFP, Tricistronic vectors: (e) HOXB4-2A-MGMT-IRES-eGFP, (f) MGMT-2A-HOXB4-IRES-eGFP. The expression of the cassette is under the control of the human PGK promoter. The central polypurine tract (cPPT) is located upstream from the transgene and the posttranscriptional regulatory element of woodchuck hepatitis virus (WPRE) is placed downstream of the transgene. RSV, Rous sarcoma virus; SD, splice donor, SA, splice acceptor; Gag, deleted gag region; RRE, Rev-responsive element; LTR, long terminal repeat; IRES, internal ribosome entry site sequence from encephalomyocarditis virus (EMCV); 2A, sequence from foot-and-mouth disease virus; eGFP, enhanced green fluorescent protein; MGMT, O6-methylguanine DNA methyltransferase (proline 140 lysine mutant); HOXB4, homeobox transcription factor.

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