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Table 1 E1 phosphate sites identified by MS analysis and site characteristics. Phospho-amino acid positions identified in this study are shown in the left column, followed by the sequence of the surrounding amino acids. The phosphorylated position is highlighted in bold type. The NetPhos2.0 and NetPhosK scores for each phospho-amino acid is shown. 1.0 is the maximum score, and 0.5 is the default threshold for likely phosphorylation. The kinases predicted by the NetPhosK algorithm are shown. Where there is no NetPhosK score, that position was not predicted and the kinases were identified by manual analysis and comparison to published consensus sequences [44]. *, phosphate sites identified by MS and not previously known; **, phosphate sites identified by MS, confirming previously known sites.

From: A phosphorylation map of the bovine papillomavirus E1 helicase

Amino Acid

Sequence

NetPhos Score

Predicted Kinase

NetPhosK Score

Ser 48**

VESDRYDS QDEDFVD

0.997

CK2 ATM DNAPK RSK

0.64 0.58 0.56 0.52

Ser 94*

VLGSSQNS SGSEASE

0.926

CK2

 

Ser 95*

LGSSQNSS GSEASET

0.993

CK2

0.59

Ser 100*

NSSGSEAS ETPVKRR

0.333

CK1, CK2

 

Thr 126*

NEANRVLT PLQVQGE

0.959

p38MPK

0.52

Ser 305*

AQTTLNES LQTEKFD

0.253

DNAPK

0.53

Ser 584**

LIDEEEDS EEDGDSM

0.998

CK2 CK1

0.69 0.53